Evaluation of anti-inflammatory effects of some terpenoids isolated from Wormwood (Artemisia vulgaris Asteraceae)

Abstract

    Introduction: In Viet Nam, Wormwood (Artemisia vulgaris - Asteraceae)  is a medicinal and aromatic bitter herb frequently used in traditional medicine since ancient times. However, the study of  Wormwood is still limited. In this research, we evaluate the anti-inflammatory effects of some terpenoids isolated from Wormwood: NC11 (Dehydromatricarin), NC12 (Moxartenolid), NC16 (Santamarin)  to contribute to creating new materials from natural sources which have good therapeutic and few side effects.

    Materials and Methods: Materials: Three compounds: NC11,NC12, NC16 were  isolated from Wormwood (Artemisia vulgaris - Asteraceae); Methods: The anti-inflammatory effects were studied using carrageenan-induced paw edema model in mice. Paw volume of mice was measured before injecting 1% carrageenan. Three hours after carrageenan injection, measure paw volume again and calculate the edema percentage. Control group was given distiled water, while experiment groups were given NC1, NC2 and positive control group received diclofenac 5mg/kg PO. The edema percentage was determined the next day, and the procedure continued in 6 days after inflammation inducing.

     Results and Discussion: Three tested compounds are all anti - inflammatory. The anti-inflammatory effects of Moxartenolid and Santamarin are similar.

     The anti - inflammatory effects of NC12 and NC16 are stronger than NC11. The anti - inflammatory effect of NC2 is 117% in comparison with 5mg/kg diclofenac.

    Conclusion: The research has successfully proved the anti-inflammatory effects of two pure natural compounds isolated from Wormwood (Artemisia vulgaris - Asteraceae), especially NC2, which is a promising compound for anti - inflammatory drug research and development.