Simultaneous determination of bromhexine hydrochloride and salbutamol sulphate in syrup by the derivative spectrophotometric method

Abstract

    In this study, the spectrophotometric method was used to simultaneously identify bromhexine hydrochloride and salbutamol sulfate in medicine syrup. The study was carried out by transforming the UV absorption spectrum of bromhexine and salbutamol using second derivatives. The results of the second derivative spectrums showed that the absorption was zero at wavelengths of 240.4nm and 365.4nm for bromhexine and salbutamol respectively. Therefore, the determination of bromhexine and salbutamol was carried out at wavelengths of 365,4nm and 240,4nm respectively. The method has been validated according to ICH guidelines. The linear curves obtained by the second derivative spectral method show that linearity in the range of 20 - 80μg/ml for bromhexine and 10 - 40μg/ml for salbutamol with the sensitivity values of bromhexine and salbutamol of 0.141μg/ml and 0.369μg/ml, respectively. This method showed the high values of recovery and accuracy for bromhexine and salbutamol. The analytical value of the intraday analysis was 98.57 - 99.86%, while the RSD of repeatability was 0.25 - 0.94%. The interday analytical values for correctness ranged from 99.80% to 101.50% and the RSD of repeatability was from 0.15% to 0.66%. The results of the research were suitable for routine analysis of bromhexine and salbutamol in the combined dosage form of syrup.