Production of rabbit IgG antibodies against excretory/sesecretory antigenantigen from Fasciola gigantica

Abstract

   Fasciolasis is becoming a global health problem. In particular, Vietnam is one of the countries with the highest circulation. In Vietnam, the disease is caused by the parasite Fasciola gigantica. The most commonly used diagnostic method is serological testing for the detection of fluke-specific IgG antibodies. Because IgG exists in the blood for a long time, this method has little value in determining the time of disease development and after treatment. In order to overcome the above-mentioned weakness of serological testing, we aim to detect the excretory/secretory antigen (E/S Ag) in blood and feces by using antibodies. The objective of this study is to use the F. gigantica E/S Ag to produce rabbit IgG. IgG was purified using 45% Ammonium sulfate precipitation and G-protein affinity chromatography. The IgG obtained is a prerequisite for the development of a diagnostic kit for the detection of F. gigantica infection on human and animal through the detection of antigens in blood and feces.