MULTIRESIDUE ANALYSIS FOR TWENTY-FOUR POLYCYCLIC AROMATIC HYDROCARBONS IN EDIBLE OILS BY ULTRASOUND-ASSISTED EMULSIFICATION MICROEXTRACTION AND SOLID-PHASE EXTRACTION CLEAN-UP FOLLOWED BY GC-MS/MS

Abstract

In this study, an analytical method for simultaneous determination of 24 polycyclic aromatic hydrocarbons (PAHs) comprising of 16 Environmental Protection Agency (EPA) priority PAHs and 15+1 European Union (EU) priority PAHs in edible oil was proposed by gas chromatography-tandem mass spectrometry (GC-MS/MS). An efficient ultrasoundassisted emulsification microextraction (USAEME) method was adopted by using dimethyl sulfoxide (DMSO) as extracting solvent followed by solid-phase extraction (SPE) clean-up. Under optimum conditions, the calibration curves showed a good linearity in the range of 1 to 50 μg/L with correlation coefficients greater than 0.995. The limit of detections (LODs) for 24 PAHs were 0.12 – 0.32 μg/kg. Using the spiked oil sample at three levels, the mean recoveries were 81 – 115% and the relative standard deviations (RSDs) in term of reproducibility were below 17.7%. Trueness assessment was further participated in international olive oil proficiency test (FCCE1-OIL22-06145) organized by the Food Analysis Performance Assessment Scheme (FAPAS) with satisfaction result (z-score < 2). The method was successfully applied to real oil samples and the results suggested that an extension to largescale study on the PAHs contamination in edible oil in Vietnamese market is encouraged.