EFFECTS OF CELLULASE ENZYME AND MICROWAVE ON POLYSACCHARIDE EXTRACTION FROM Enteromorpha sp. ALGAE

Abstract

This study focused on the extraction of polysaccharides (PS) from Enteromorpha using two methods: enzyme-assisted extraction (EAE) and microwave-assisted extraction (MAE). The findings determined that the optimal conditions for MAE were a microwave power of 420 W, an extraction time of 3 min, and a solid-to-liquid ratio of 1:20 w/v. For EAE, the best results were obtained with a solid-to-liquid ratio of 1:20 w/v, pH of 5.0, an extraction temperature of 50 °C, and an extraction time of 4h. The polysaccharide yields were 205.4 ± 0.64 mg/g dry matter for MAE and 147.9 ± 0.13 mg/g dry matter for EAE.