In vitro propagation and factors affecting the growth of Amomum longiligulare T.L. Wu in Quang Ninh Province

Abstract

Amomum longiligulare T.L. Wu is a high-value medicinal plant with significant economic importance, particularly well-suited for under-canopy cultivation. In vitro propagation experiments were conducted on MS medium supplemented with different concentrations of BAP and NAA. The results indicated that MS medium supplemented with 1.5 mg/l BAP and 0.5 mg/l NAA produced the highest shoot multiplication rate and optimal root induction. At the nursery stage, a substrate mixture of alluvial soil, rice husk biochar, and organic humus (in a 1:2:1 ratio) achieved the highest survival rate (94.32%). Under field conditions, experiments on canopy cover and fertilization regimes showed that the plants performed best under 30–60% canopy cover with a fertilization regime consisting of a basal application of 4 tons/ha of organic microbial fertilizer, followed by 2 tons/ha of organic microbial fertilizer and 1 ton/ha of NPK in the second and third years. This study established an appropriate in vitro propagation and cultivation protocol for A. longiligulare in Quang Ninh Province, contributing to germplasm conservation, sustainable under-canopy economic development, and a stable supply of high-quality medicinal raw materials for the market.