COMPARISION OF LIPID EXTRACTION EFFECTIVENESS FROM Sargassum oligocystum ALGAE BY ENZYME AND ULTRASOUND METHODS

Tóm tắt

This study aimed to examine the effects of enzyme and ultrasound on lipid extraction from Sargassum oligocystum algae. The enzyme-assisted extraction conditions of lipid with the enzyme included: enzyme ratio (0, 0.5, 1, 1.5, and 2 v/w), pH (3.5, 4, 4.5, 5, and 5.5), temperatures (35, 40, 45, 50, and 55 ℃) and extraction time (60, 90, 120, 150, and 180 min). The investigation of lipid extraction parameters from S. oligocystum algae using ultrasonic waves, such as material/solvent ratio (1/5, 1/10, 1/15, 1/20, and 1/25 w/v), time (5, 10, 15, 20, and 25 min). The results showed that the suitable conditions for lipid extraction with Viscozyme L were an enzyme concentration of 1.5%, pH 4.5, at 50 ℃ for 150 min. The results show that the recovery efficiency of lipid extraction from S. oligocystm algae using shaking method combined with Viscoenzyme L treatment (48.17 ± 0.91%) was higher than the that of ultrasound method (46.88 ± 1.03%).