RESEARCH OF CHITOSAN HYDROLYSIS BY CELLULASE TO PRODUCE CHITOOLIGOSACCHARIDE

Abstract

To increase the water solubility of chitosan and the potential application for many good products for human health is the primary objective of this study. Cellulase is non-specific enzyme used in this study, the reducing sugar content is determined using the DNS method, the molecular weight of chitooligosaccharide (COS) is determined by gel permeation chromatography (GPC). The research shows that the suitable parameters for the hydrolysis
process include: temperature (50 oC), pH (5.5), substrate concentration (0.8%), enzyme
activity (7 UI/g), hydrolysis duration (150 minutes). Average molecular weight of COS is
4,325.8 (Da). The research results may be applied to produce COS.