STUDY ON PRODUCTION OF BROMELAIN POWDER FROM PINEAPPLE WASTES

Abstract

This study was conducted to produce bromelain powders from pineapple wastes by spray drying. The results showed that the suitable carrier agent was skim milk at a concentration of 10% (w/v). To obtain the highest activity of bromelain enzyme, the drying process was carried out at inlet air temperature of 110 °C, outlet air temperature of 60 °C, feed flow rate of 288 mL/h (8 rpm). The obtained bromelain powder had activity of 1542,550 ± 98,384 (UI/g), dynamic parameters Km = 2,031 mM and Vmax = 0,054 mM/min with casein substrate, hydrolysis activity at pH 7 and 40 oC. Bromelain powder was stored at 0 °C in aluminum bags and can be kept 50% activity after 20 days.

Keywords: Enzyme, bromelain, membrane processing, pineapple wastes, spray drying