Screenning and determination of polyhydroxyalkanoate production by Bacillus sp. strains

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by microbes as reserve material when they were cultured in a limitation of nutrients and excess C source mediums. PHAs can be synthesized by various bacteria including gram positive and gram-negative bacteria. Among the PHA-producing gram-positive bacteria, Bacillus sp. produce and accumulate various monomer compositions of PHAs. In this study, about 83,3% of strains (05/06 strains) can accumulate PHAs production. Bacillus sp. DV01 accumulated a amount of PHA 23,9% of CWD after 96h when cultured in modified basal medium containing 1% (w/v) glucose. Bacillus sp.  DV01 was identified by sequecing the gene coding for 16S rRNA. BLAST results showed the obtained 16S rRNA sequence of this strain revealed 100% with the Bacillus megaterium and so-called Bacillus megaterium DV01.