The presence of highly pathogenic avian influenza viruses in live poultry selling at some markets in Khanh Hoa province

Abstract

The study was conducted in Khanh Hoa province, there were 140 oropharyngeal swabs and fecal samples of chickens/ducks collected in the live-poultry markets to detect the presence of influenza A virus, subtype H5N1, H5N6, H5N8, and H7N9. Realtime quantitative reverse transcription - PCR (RT-qPCR) was used to detect the influenza viruses. The studied results showed that the infection rate of samples with influenza A virus was 46/140 samples (32.86%), while 3/140 samples (2.14%) were positive with A/H5N1, and all the rest samples were negative with H5N6, H5N8, and H7N9. The rate of positive samples with influenza A virus in the Vinh Hai market was the highest with
22/35 samples (62.86%), of which, 3 samples were positive with H5N1. There were 8/35 samples (22.86%) in Thanh, Cam Hai and Song Can markets positive with influenza A virus, and all the rest samples were negative with A/H5 and A/H7. Influenza A viruses were found in all studied months,
the highest positive rate was in April (42.86%) and the lowest positive rate was in March (28.57%). In March and April, the influenza A/H5 and A/H7 viruses were not found, but H5N1 viruses were identified in May (2.14%). The results of this study would serve as a basis for Khanh Hoa province in
marking appropriate prevention measures against the influenza A virus.