Determining esophageal worm species caused disease in dogs in Viet Nam by molecular biology method

Abstract

In order to identify accurately the esophageal parasite worm species in the farmed dogs in Viet Nam, a study on this topic was conducted in 2020 - 2022. The studied results showed that the esophageal parasite worm species in dogs in Dien Bien province belonged to the genus Spirocerca, species Spirocerca
lupi Rudolphi, 1809. In this paper, for the first time, Spirocerca lupi parasitic worms in Viet Nam was identified by molecular biology methods, through sequence analysis of mitochondrial cytochrome c oxidase subunit I (CO1) and comparison with sequencing results of this gene published on GenBank. As a result,
the mitochondrial genomic DNA sequence of cytochrome c oxidase subunit I (CO1) of Spirocerca lupi esophageal worms was identified with the length was about 500 bp. The similarity level of sequences of Spirocerca CO1 gene collected in Dien Bien was 100% on genetics and having evolutionary relations was similar to the CO1 gene sequences of Spirocerca lupi in China and Israel.