Study on the circulation of DIV1 virus in farmed shrimp in Viet Nam

Abstract

This study was conducted to detect and evaluate the circulation of DIV1 in the farmed shrimps in our country. The samples from shrimp farms (white leg shrimp, black tiger shrimp, giant freshwater shrimp), live feeds for broodstock, imported quarantine shrimp samples, conservated shrimp samples in the aquatic laboratories, wild shrimp and crustacean samples, and suspected diseased shrimp were collected for this study. The number of these collected samples were representative for locations, seasons, farming model and scales. A repeated cross-sectional study method was used with a 95% confidence level and 4% of
design prevalence of the DIV1 infection in the farmed shrimps. The results of DIV1 analysis by real-time
PCR and nested PCR techniques revealed that there were 3,531 samples collected, including 2,067 shrimp
samples from rearing households, 160 polychaetes samples, 131 wild shrimp and crustacean samples, 230 conserved samples in the laboratories, 360 samples of quarantined shrimp, 583 disease suspected samples examined. As a result, 100% samples were negative with DIV1. Thus, within the study’s scope, subjects and time, we confirmed that the circulation of the DIV1 virus was not found in Viet Nam.