Determining infection rate and antibiotics resistance of Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA) bacteria iolated from fresh cow milk

Abstract

This study was conducted to determine the prevalence, antibiotic resistance profile, and molecular characteristics of Staphylococcus aureus isolated from 400 fresh cow milk samples collected in Ba Vi district, Ha Noi City. The studied results showed that there were 21/400 (5.25%) milk samples positive with S. aureus. The isolated S. aureus strains in this study resisted at the highest rate to penicillin (95.24%), followed by ampicillin (76.19%), and cefazoline (61.9%). On the contrary, they resisted at the low rate to chloramphenicol, clindamycin, ciprofloxacin, and norfloxacin (with the same resistance rate of 4.76%). The
identification result by PCR technique revealed that 5/21 (23.81%) of the isolates were methicillin-resistant S. aureus (MRSA), and all of these MRSA strains carried mecA gene. Notably, 3/5 (60%) MRSA isolates harbored sea gene, 1/5 (20%) strains carried seb gene, and 1/5 (20%) strains carried sed gene. The sec, see, pvl, and mecC genes were not detected in all MRSA isolates.