Isolation and determination of antibiotic resistance of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in shrimp

Abstract

Acute hepatopancreatic necrosis disease (AHPND) in shrimp caused by Vibrio parahaemolyticus is one of the most important diseases for the aquaculture industry on the worldwise. In this study, 50 shrimp samples suspected with AHPND infection were collected at shrimp farms in Hai Hau district, Nam Dinh province for isolating V. parahaemolyticus. The studied results showed that 43/50 (86%) samples were positive with V. parahaemolyticus. Eight out of forty-three (18.6%) isolates were found to habour the gene encoding pirA toxin causing AHPND. These 8 V. parahaemolyticus isolates exhibited the highest resistance rates to ampicillin (100%) and cefotaxime (100%), followed by ciprofloxacin (37.5%), chloramphenicol (37.5%), tetracycline (25%), and the lowest resistance rate
to gentamicin (12.5%). All isolates were resistant to 2 or more antibiotics with 6 resistance patterns. Notably, 2 out of 8 (25%) isolates were identified as multidrug-resistant strains and one isolate was capable of resisting all the tested antibiotics