Research on production and evaluation for prevention, treatment efficacy of egg yolk immunoglobulin against 3-1E antigene of chicken coccidia

Abstract

This study aimed at production and evaluation for prevention-treatment efficacy of egg yolk immunoglobulin against 3-1E antigene of chicken coccidia. The experimental chickens were immunized by 3 repeated injection times, each injection dose was 1ml solution containing 50 μg of recombinant 3-1E antigene, 300 μl of Freund adjuvant and just enough PBS. By using lyophilized method, 6-7g of powder from 1 egg were obtained with antibody content was around 12mg/g. After reverting with PBS (1: 9, w/w) the solution presented specific antibody titer reaching 1/128.000. Using egg yolk powder containing antibodies against recombinant 3-1E antigene showed good results in prevention and treatment of coccidiosis that caused by Eimeria acervulina, E.tenella, E.maxima in chicken, such as: reducing morbidity, mortality, oocyst shedding as well as increasing recovery rate and growth performance.