Molecular epidemiology of Mammalian orthoreovirus from domestic dogs in Ho Chi Minh City, Viet Nam

Abstract

Mammalian orthoreovirus (MRV) is a pathogen which can cause diseases in human and many species of animals. Dogs are companion animals which often expose to human, so infection risks from this species is very high. The prevalence of MRV in dogs has been reported in many countries. However, there is currently lack of MRV information in dogs in Viet Nam. The objective of this study was to investigate and characterize MRV circulating in Viet Nam. A total of 80 fecal swab samples from dogs (30 samples from diarrheic dogs, 50 samples from healthy dogs) were detected for MRV by RT-PCR. The studied result showed that prevalence of MRV in the diarrheic dogs was higher than prevalence of MRV in the healthy dogs. A total of four MRV strains were successfully isolated on cell A72/cSLAM. Phylogenic analysis showed that one Vietnamese MRV strain belonged to genotype 1 (MRV-1) and three Vietnamese MRV strains belonged to genotype 3 (MRV-3). Vietnamese isolates - MRVs-3 were high identity in comparison to the Chinese MRV isolated from pigs. The Vietnamese isolates - MRV-2 was high identity in comparison to the Chinese isolates - MRV, which caused disease in human. These results indicated that there was transmission of MRV among different animals, also spread between animals and human. The Vietnamese isolates – MRV can be potential candidates for development of vaccine or probiotics in Viet Nam.