The application of RT-PCR for diagnosing canine coronavirus in domestic dogs

Abstract

CCoV is a pathogen causing enteritis in dogs in many countries around the world. The application of fast, accuracy and timely diagnostic techniques is essential. In this study, RT-PCR technique was established and applied for diagnosing and investigating the prevalence of CCoV-II in the domestic dogs raising in Ha Noi and surrounding area. The studied result showed that RT-PCR with a pair of 2bF- 2bR primers (detected 1copy/μl) was more sensitive in comparison with S5-S6 primers (detected 10 copies/μl) in identifying CCoV-II. The specificity of both primers 2bF-2bR and S5-S6 was high for detecting CCoV, and not to detect the CDV and CPV-positive samples. The prevalence of CCoV-II in Ha Noi and surrounding area was 30%, the infection rate of the exotic dog breeds (77.23%) was higher than that of the indigenous dog breeds (22.27%) and prevalence in the dog group from 2 months old to less than 5 months old was the highest (68.18%).