Study on some biological characteristics of Canine distemper virus isolated in the North, Viet Nam

Abstract

Canine distemper caused by canine distemper virus (CDV) is a contagious, often fatal, multi-systemic viral disease, affecting the respiratory, gastrointestinal and central nervous systems of dogs. In this study, 12 canine distemper virus strains were isolated on Vero - DST cell. Cytopathic effects of CDV infection were observed at 24 hours to 36 hours after infection, reaching a high level of destruction at 48 hours to 60 hours after infection and Vero-DST cells were completely destroyed after 96 hours. The titer of isolated canine distemper virus ranged from 6.67x104 TCID50/ml to 3.16x105 TCID50/ml. Virus titer in the cells were greater than the extracellular virus titer. Virus growth in Vero-DST cell culture was a continuous process in which the viral titer was highest at 48 hours to 60 hours after infection