Isolation and identification of biological characteristics of Canine Coronavirus in domestic dogs in Ho Chi Minh City

Abstract

The objective of this study aimed at identifying, isolating and determining the biological characteristics of the Canine Coronaviruses, circulating in the domestic dogs in Ho Chi Minh City. The studied result indicated that the prevalence of CCoV-II in the domestic dogs in Ho Chi Minh City was 10% (5/50). All the detected CCoV-II belonged to subtype: CCoV-IIb. This was predominant subtype, circulating in the domestic dogs. The type: CCoV-I was not found in the domestic dogs. One CCoV-IIb strain was successfully isolated. Genetic analysis of this isolated CCoV-IIb strain showed that it possessed close relationship with the Chinese CCoV-IIb strains in the wild animals. The isolated CCoV-IIb strain grew well in fcfw-4 cells rather than in the other cell lines. There was not clinical signs in the experimental infection dogs with CCoV-IIb strain, but this virus strain generated immune response in the infection dog. The isolated CCoV-IIb was a potential strain for researching, developing vaccine and other biological products for diagnostics.