Detection and analysis of the genetic characteristics of Canine Kobuvirus in domestic dogs in Ho Chi Minh City

Abstract

Canine Kobuvirus (CKoV) is a pathogen relating to enteritis in the domestic dogs. Currently, prevalence of CKoV in dogs has been reported in many countries. However, information on CKoV in the domestic dogs is lack in Viet Nam at present. The objective of this study was to investigate and charaterize CKoV, circulating in Ho Chi Minh City, Viet Nam. A total of 80 fecal swab samples from dogs (30 samples from the diarrheic dogs, 50 samples from the healthy dogs) were detected for CKoV by RT-PCR technique. The studied result showed that the prevalence of CKoV in the diarrheic dogs was 10% higher than that in the healthy dogs (4%). However, there was no statistically significant differences in prevalence of CKoV from the diarrheic and health dogs (P>0.05). The infection of CKoV with other enteritic viruses (CPV, CDV and MRV) was commonly found in the domestic dogs. It indicated that CKoV was a minor pathogen in enteritis in dogs. Genetic analysis showed that 5 detected CKoV strains presented high similarity level to Korean and British CKoV strains (95.8%-99.5%). This is the first report on CKoV infection in the domestic dogs in Ho Chi Minh City.