Comparing the diagnositic and isolation methods for Feline panleukopenia virus (FPV)

Abstract

The objective of this study aimed at comparing the diagnositic and isolation methods for Feline panleukopenia virus (FPV). The studied results showed that the sensitivity level of PCR test were higher than that of ELISA test (47.06% and 40.20%, respectively). However, the time of diagnosis by PCR methods were longer than that of the ELISA test. Virus quickly replicated in such Crandall-Reese Feline Kidney (CRFK) cells and caused typical cytopathogenic effect
(CPE) (cells clumped and sloughed from the bottoms of culture flask). FPV caused CPE in CRFK cells as early as 4 hours post inoculation (hpi) and after 32 hpi, almost of cells were detached from the surface of the culture flasks. The titer of virus (TCID50/25µl) reached a peak at 32 hpi and over the period of 4 hours (TCID50/25µl fluctuation from 108 to 1011).