Study on cloning and expressing trh gene (thermostable direct hemolysinrelated hemolysin) of Vibrio parahaemolyticus encoding antigen caused thermostable hemolysin in snapper (Sciaenops ocellatus)

Abstract

n this study, we have successfully cloned and expressed the trh gene encoding for a thermostable
direct hemolysin-related hemolysin of Vibrio parahaemolyticus, which caused ulcer disease in snapper
(Sciaenops ocellatus) raising in coastal areas of Thua Thien-Hue province. The full-length of trh gene
was 462 bp (including start codon ATG and termination codon TGA). Similarity level of this gene
sequence was 100% compared to the trh gene sequence published on the GenBank (accession
number KP836471.1). The trh gene created an entire polypeptide sequence consisting of 154 amino
acids and its similarity level was 100% compared to the polypeptide chain published on the GenBank
(accession number ALZ44850). The result of analyzing SDS electrophoresis showed that 6xHis-TRH
fusion protein had a molecular weight of approximately 21 kDa (including 3.7 kDa of 6xHis fusion tail).