Study on isolating Canine Parvovirus in northern provinces, Viet Nam

Abstract

56 rectal swab samples collected at veterinary clinics in Thai Binh, Ha Nam, Hai Duong provinces, Viet Nam from August 2019 to Febuary 2020 having the positive test result with CPV by PCR method was used for isolating CPV on CRFK and MDCK cell lines. As a result, 4 CPV strains causing cyto-pathogenic on CRFK cells were selected, this was the specific cell line to CPV isolation. The results of determining biological characteristics of CPV isolates on CRFK cell lines showed that
cyto-pathogenic effect (CPE) caused by CPV of the 7th generation, appeared from 36 hours to 48 hours, post-infection, CPE reached the highest peak from 60 hours to 72 hours, post-infection, and
the cells were totally destroyed after 84 hours, post-infection. The titers of 4 CPV strains were high, reaching from 1.69x106 to 2.73x107 TCID50/ml