Evaluation on foot and mouth virus inactivation efficacy of binary ethylenimine (BEI) in commercial vaccine production

Abstract

Titration curve, complete inactivation time and safety efficacy were assessed for defining the
concentration of binary ethylenimine (BEI) in inactivation of foot-and-mouth disease virus, type O in
commercial vaccine production. The inactivation rate ranged from 0.2 to 0.8 lg TCID50/ml/hour were
obtained using BEI with morality 0.5, 1, 1.5 and 2 mM. The complete inactivation time reduced
corresponding to the higher concentration of BEI, it was occurred at 49 hours post inactivation using
0.5 mM BEI and shortened to less than 22 hours at concentration above 1 mM. Safety assessment on
BHK21 cells revealed the compatible results. The durations to reach safety target were 32 and 20 hours
after inactivation using BEI with concentration of 0.5. and 1 mM, respectively. Morover, there were no
significant different results achieved when increasing concentration of BEI. We recommend using BEI
with morality 1 mM for optimal inactivation efficiency in FMD industrial vaccine production scale