Cloning and expression of gene encoding P102 antigen of Mycoplasma hyopneumoniae in E. coli BL21 (DE3)

Abstract

In this study, we successfully cloned and expressed a fragment of gene encoding P102
antigen of Mycoplasma hyopneumoniae isolated from lung samples of pigs raised in Thua ThienHue province, Viet Nam. The fragment of gene encoding P102 antigen was cloned into plasmid
pGEM®-T Easy and expressed with vector pET200/D-TOPO in Escherichia coli BL21 (DE3)
cells. The studied results showed that the fragment length of gene encoding P102 antigen was
912 bp, encoding a polypeptide chain with 304 amino acid residues, and 100% similarity to the
polypeptide chain of P102 antigen in GenBank (accession number AAZ44196.1). Results of
SDS-PAGE electrophoresis showed that molecular weight of 6xHis-P102 recombinant protein
was about 38 kDaIn this study, we successfully cloned and expressed a fragment of gene encoding P102
antigen of Mycoplasma hyopneumoniae isolated from lung samples of pigs raised in Thua ThienHue province, Viet Nam. The fragment of gene encoding P102 antigen was cloned into plasmid
pGEM®-T Easy and expressed with vector pET200/D-TOPO in Escherichia coli BL21 (DE3)
cells. The studied results showed that the fragment length of gene encoding P102 antigen was
912 bp, encoding a polypeptide chain with 304 amino acid residues, and 100% similarity to the
polypeptide chain of P102 antigen in GenBank (accession number AAZ44196.1). Results of
SDS-PAGE electrophoresis showed that molecular weight of 6xHis-P102 recombinant protein
was about 38 kDa