Bacillus spp. isolation from soil and chicken feces in Tra Vinh province

Abstract

From 35 soil samples and 35 fecal samples of chicken farms collecting at 2 districts in Tra
Vinh province, 62 Bacillus spp. bacteria strains were isolated. These isolates were analyzed
further for Bacillus spp. characteristics by physio-biological tests with the following criteria:
negative with lecithinase, positive with catalase, amylase, cellulose, VP (Voges-Proskauer)
and temperature tolerance (being able to grow at 500C). The studied results showed that there
were 7 isolates met the standards of probiotic criteria.
The result of sequencing 16S rRNA (using BLAST alignment with the known sequences on
the NCBI GenBank) indicated that nucleotic similarity level of P6.N and P21.N strains compared
to those of Bacillus coagulans and Bacillus subtilis was 100%, respectively.