Isolation and determination on biological characteristics of Clostridium perfringens in chickens raised in some provinces of Viet Nam

Abstract

Necrotic enteritis is one of the most important enteric diseases in poultry and causes high economic loss to the livestock industry. The recent studies have shown that NetB toxin of C. perfringens is a critical virulence factor in the pathogenesis of necrotic enteritis in chickens. In this study, we collected 300 fecal and 300 intestinal samples from the necrotic enteritis suspected chickens in the provinces: Thai Binh, Nam Dinh, Binh Dinh, Khanh Hoa, Can Tho, and Tien Giang to isolate C. perfringens. The studied result showed that 101/300 (33,67%) of fecal samples and 171/300 (57%) of intestinal samples contaminated with C. perfringens,
respectively. The results of determining the toxin type by multiplex-PCR indicated that 272/272 (100%) of C. perfringens isolates belonged to type A. The PCR for detection of netB gene showed that 69/272 (25.37%) of C. perfringens isolates harbored netB gene.