Study on antibiotic resistance phenotype and genotype of Escherichia coli producing ESBL from pig waste

Abstract

Antimicrobial resistance bacteria is a threat to public health in the worldwide and transmission of the
resistant genes is the most important antibiotic resistance mechanism of bacteria. A total of 43 E. coli strains
producing ESBL isolated from the pig feces samples in Bac Ninh province were used for analyzing their
gene sequences by Illumina Nextera XT method using v3 protocol of MiSeq kit. The studied result showed
that the antibiotic resistance genes identified from the above isolated bacteria strains included blaCTX-M-55
(22/43), blaCTX-M-14 (10/43), blaCTX-M-27 (5/43), blaCTX-M-15 (2/43) and blaOXA10 (4/43). There was
1 isolate producing ESBL carried both blaDHA, AmpC genes (the gene resisted beta-lactam antibiotics, C
group). There were 18/43 E. coli isolates carried the plasmid-mediated mcr-1 gene (resisted colistin). Of
which, there were 4 isolates carried the mcr-3 gene (resisted colistin), 2 isolates had mutations in the prmB
gene (p.V161G). Most of the E. coli strains (41/43) producing ESBL harbored plasmid-mediated genes
resisted beta-lactam antibiotics (quinolone group) (qnrS1 and/or qnrB6 and/or qnrB19) and/or quinolone
resistance due to mutations. These resistant genes could be transmitted following the horizontal or vertical
ways among different bacterial strains/species. This is one of the witness on spreading the antibiotic
resistance genes of E. coli strains isolated from the pig waste in Viet Nam