STUDY ON DETERMINATION OF 6 ANTIBIOTICS IN WASTEWATER COLLECTED FROM SEVERAL HOSPITALS BY ULTRA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY TANDEM WITH HIGH RESOLUTION MASS SPECTROMETRY (UPLC- ORBITRAP MS)

Abstract

This work has introduced method UPLC- Orbitrap MS to determine six antibiotic compounds
(Ciprofloxacin, Cephalexin, Moxifloxacin, Clarithromycin, Levofloxacin) in wastewater collected from
several hospitals in Vietnam. The chromatographic procedure used Zorbax-eclipse XDB C18 100 x 4,6
mm, 3,5 µm (Agilent) and 0.1% FA in deionized water and MeOH as mobile phases. Selected ion
monitoring (SIM) mode was used for qualification and qualitication of target analytes. The parameters
such as: temperature column, mobile phases, gradient, flowrate were optimized. The investigated
compounds were cleanup from match-matrix based on SPE extraction using HLB column(supelco). The
spiked experiments were carried out for method evaluation through several parameters, such as:
repeatability, recovery (85,2 ± 10,1% - 107,6 ± 3,5%), linearity, MDL 0,03 ng/L (Cephalexin,
Amoxicillin) – 0,07 ng/L (Clarithromycin, Ciprofloxacin, Levofloxacin) và LOQ nằm trong khoảng từ
0,10 ng/L (Cephalexin) – 0,23 ng/L (Clarithromycin). Applying this method, 30 real samples collected
from five hospitals in our country were analyzed. It can be clearly seen that their systems of treatment
wastewater have been operated effectively.