AN OVERVIEW OF IN VITRO TESTS USED FOR PROBIOTIC SCREENING LACTOBACILLUS SPP. POTENTIAL

Abstract

Probiotics are no longer a strange concept to many people. In general, the benefits of adding probiotics to food, pharmaceuticals for humans, or animal feed in particular have been proven through many studies. The process from a probiotic strain to an effective probiotic product requires a lot of testing. Especially, in vitro tests are considered as a necessary starting point when screening a potential probiotic strain. In vitro tests are the basis for assessing whether a strain can be used for production or not, and also provide a preliminary basis for considering which probiotic strain serves the most optimal field. Commercial probiotics today are often sourced alone or from a combination of many different bacterial strains from the genera Lactobacillus, Bacillus, Bifidobacteria, etc. Among them, the most researched are Lactobacillus probiotic strains. However, there has not been any study addressing specific standards in in vitro screening for potential probiotics Lactobacillus. In this review, we focus on analyzing and synthesizing in vitro assays for Lactobacillus probiotic screening from research works over the last few years, to providing necessary standards and specific methods for in vitro testing, apply to selecting potential probiotics Lactobacillus strains.