A RESEARCH ON GENETIC DIVERSITY OF BASIL, IDENTIFICATION OF HEREDITARY RELATION BETWEEN LANG BASIL AND OTHER BASIL VARIETIES/SPECIES USING PCR - RAPD TECHNIQUE.

Abstract

            Basil (Mentha aquatica) belonging to the family Labiateae has a diverse native genetic resource in Vietnam. Using 15 random RAPD primers, a total of 2,435 bands have been obtained, which are classified into 47 polymorphic band types (44.3%) and 59 monomorphic band types (55.7%). The coefficient of genetic similarity among 32 studied varieties has ranged from 0.72 to 1. Among them,11 Lang basil varieties collected in different locations have close genetic variation ranging from 0,00 to 0.11. At the 80% genetic similarity, 32 varieties have been divided into 5 different groups. Based on the results of isolated genetic groups, 11 Lang basil varieties have been selected for gene sequencing (with high conservation) in order to identify molecular markers, document genetic resource of endemic Lang basil and register on the world gene banks.