CARBOPLATIN MEASURING METHOD WITH FREEZED DRY SVP CARBOPLATIN BY HPLC.

Abstract

                The high performance liquid chromatography is a fast, sensitive and accurate method. Therefore, we made carboplatin measuring method with freezed dry SVP Carboplatin 50 mg and 150 mg with liquid chromatography with chromatographic conditions as follow: UV detector at 230 nm wave length; column: RP 18 (250x4.6 mm; 5 μm); mobile phase: water: acetonitrile (20:80); flow rate: 1ml/min. This method has been evaluated to meet requirements of linear range: linearity range: 0.4-1.4 ppm (R2 = 0.9999); the detecting limit: 2.012 μg/ml; the quantitation limit: 6.099 μg/ml; precision: RSD = 0.96%; accuracy (recovery rate): 100.67%, RSD = 0.21%.